New CRISPR technique confirms methylation's role in gene silencing

New CRISPR method removes DNA methyl groups to reactivate silenced genes, offering safer genetic disease treatment.

Why it matters

  • Confirms methylation directly silences genes.
  • Offers safer genetic disease treatment, avoiding DNA cuts that can cause cancer.
  • Could treat diseases like Sickle Cell by reactivating fetal globin gene.

By the numbers

  • Study published in Nature Communications.
  • Collaboration between UNSW Sydney and St Jude Children's Research Hospital.
  • Tested in human cells in lab so far.

The big picture

  • Part of trend towards safer gene editing techniques.
  • Potential applications beyond Sickle Cell.
  • Epigenetic editing could avoid unintended effects of DNA cutting.

What they're saying

  • Novelty is in specific demethylation of target gene.
  • Methylation can work differently in different contexts.
  • Exciting advance in gene editing technology.

Caveats

  • Work done in human cells in lab so far.
  • Needs animal testing and clinical trials before human use.
  • Methylation effects can vary by gene context.

What’s next

  • Test in animal models.
  • Move to clinical trials.
  • Explore other CRISPR-related tools.